解剖学报

›› 2011, Vol. 42 ›› Issue (6): 737-740.doi: 10.3969/j.issn.0529-1356.2011.06.004

• 神经生物学 • 上一篇    下一篇

DARPP-32在大鼠全脑的定位分布

王伟文1*SUB>;/SUB> 廖晓阳2 ; 吴俞宪1; 王庆松1 ;林杭1 ;肖莉1 ;陈良为3 ;饶志仁3   

  1. 1．成都军区总医院神经内科，成都 610083; 2.四川大学华西医院金卡病房，成都 610041;3.第四军医大学全军神经科学研究所，西安 710032
  • 收稿日期:2011-04-11 修回日期:2011-06-21 出版日期:2011-12-06
  • 通讯作者: 王伟文

Immunohistochemical localization of DARPP-32, a dopamine and cyclic-AMP-regulated phosphoprotein, in the rat brain

  1. 1.Department of Neurology, Chengdu Military General Hospital, Chengdu 610083, China;2.VIP Ward, West China Hospital, Sichuan University, Chengdu 610041, China;3.Institute of Neuroscience, the Fourth Military Medical University, Xi’an 710032, China
  • Received:2011-04-11 Revised:2011-06-21 Online:2011-12-06
  • Contact: WANG Wei-wen1

关键词: DARPP-32, 脑, 免疫组织化学, 大鼠

Abstract: Objective To study the distribution of DARPP-32 in the rat brain. BR>Methods Immunohistochemical staining techniques were applied to study the localization of DARPP-32 in neurons of the rat brain. Results The strong immunoreactivity for DARPP-32 was localized primarily in the basal ganglia and the anterior olfactory area, involving neuronal cell bodies or dendrites in the nucleus accumbens, caudate putamen, amygdaloid nucleus and fibers throughout the globus pallidus, ventral pallidum and pars reticulate of the substantia nigra. The moderate to weak immunoractivity for DARPP-32 was observed in red nucleus, septofimbrial nucleus, purkinje cells in cerebellum, medial habenula nucleus, cortex and hippocampus. DARPP-32 was present in the cytoplasm, cytomembrane and cell nucleus of labeled neuronal somata and dendrites. Conclusion DARPP-32 is widely distributed in the rat brain. It is primarily present in the neurons, which receive a dopamine input and contain dense of the D1 receptor. DARPP-32 may play an important role in dopaminoceptive neurons. BR>

Key words: Dopamine and cAMP-regulated phosphoprotein-32, Brain, Immunohistochemistry, Rat

中图分类号: